The Jackson Laboratory: Georg Blaser

Georg Blaser

Georg Blaser

Postdoctoral Research Assistant
M.Sci., Chemistry, University of Bristol, UK
Ph.D., Chemistry, Durham University, UK
Rm. 290 • +44 (0)1223 336357 • gb368@cam.ac.uk

Present Research

  1. Single Molecule and Ensemble Analysis of the Stability and Dynamics of FRET-labelled Ubiquitin
  2. Single Molecule Studies on the Conformational Space, Binding Affinities and Enzymatic Activity of FRET-labelled Ubiquitin Chains
  3. Unfolding and Folding of Yellow Fluorescent Protein (YFP) venus
  4. Single Molecule Studies on Blue Fluorescent Protein (BFP)

Previous Research

Lipid-peptide binding was analyzed using a model system. Tryptophan derivatives carrying substitutions on the 5' and 6' position of the indole ring were mixed with dimyristoylphosphatidylcholine (DMPC) lipids in chloroform and binding interactions monitored by alteration of the chemical shifts upon binding. Furthermore, in silico studies were performed to predict the effect of the electron density of the indole ring on binding and to visualize and extract binding configurations between the amino acid and lipid molecules.

Publications:

  • Li H, Shim JU, Ibrahim S, Ranasinghe R, Blaser G, Clarke R, Jackson S, Huck WTS, Abell C, Klenerman D (2010)
    "Enhanced single molecule detection under conditions of fast flow"
    Nat. Methods, submitted
  • Hsu STD, Blaser G, Behrens, C, Cabrita LD, Dobson, CM, Jackson, SE (2010)
    "Folding study of Venus reveals a strong ion dependence of its yellow fluorescence under mildly acidic conditions"
    J. Biol. Chem. 285(7):4859-4869. PDF
  • Hsu STD, Blaser G, Jackson SE (2009)
    "The folding, stability and conformational dynamics of β-barrel fluorescent proteins."
    Chem. Soc. Rev. 38:2951-2965. PDF
  • Blaser G, Sanderson JM, Wilson, MR (2009)
    "Free-energy relationships for the interactions of tryptophan with phosphocholines."
    Org. Biomol. Chem. 7:5119–5128. PDF
  • Andersson FI, Pina DP, Mallam AL, Blaser G, Jackson SE (2009)
    "Untangling the folding mechanism of the 52-knotted protein UCH-L3."
    FEBS J. 276:2625-2635. PDF
  • Blaser G, Sanderson JM, Batsanov AS, Howard JAK, (2008)
    "The facile synthesis of a series of tryptophan derivatives"
    Tetrahedron Lett. 49(17):2795-2798.PDF